detection of hydatid fluid and protoscolices antigens in sheep with hydatidosis

Detection of hydatid fluid and protoscolices antigens in sheep with hydatidosis

Hydatidosis is a disease caused by infection with the metacestode stage of the dog tapeworm,Echinococcus granulosus. This is recognised as one of the world’s most important zoonoses, affecting bothhumans and their domestic animals. In the current study, 20 sheep cystic livers or lungs were collected.Hydatid fluid and protoscolices were isolated and the soluble protein was prepared. Polypeptide ...

Immunization of Lambs against infection with Echinucoccus granulsus using protoscolices and hydatid fluid antigens

Antibody response against hydatid fluid, protoscolex and whole body of Echinococcus granulosus antigens in lambs

In this study, the immunogenicity of three types of antigens (hydatid fluid, protoscolices and whole body of E. granulosus) was investigated in lambs by ELISA. Sixteen 4–6-month-old lambs of mixed sexes were divided into 4 groups of 4 lambs (three immunized and one control group). Twelve lambs as immunized groups received 2 mg of hydatid fluid, protoscolices and whole body of E. granulosus ant...

Antibody response to glycan antigens of hydatid cyst fluid, laminated layer and protoscolex of Echinococcus granulosus

Background: Hydatid disease is characterized by long-term growth of hydatid cysts in the human. The glycan antigens have an important role in the immunology of hydatid cyst. In this study immunological reaction of host sera to different glycan antigens of the cyst, has been investigated. Methods: The antibody responses were tested to glycoprotein and glycolipid of the laminated layer (LL), c...

Evaluation of Enzyme Linked Immunosorbent Assay, Utilizing Native Antigen B for Serodiagnosis of Human Hydatidosis

Background: Hydatidosis is one of the cosmopolitan parasitic zoonoses caused by the larval stage of Echinococcus granulosus. Diagnosis of hydatidosis is still an unresolved problem. Serological tests using crude antigens for diagnosis of E. granulosus are sensi-tive, however their specificity are not satisfactory. Therefore, WHO recommended spe-cific serological methods using specific antigens,...

Evaluation of Protoscolex Antigens for Hydatidosis Detection before and after Surgery

Introduction Cystic echinococcosis (CE) is a zoonotic infection caused Echinococcus granulosus with worldwide distribution. As one of the problems that can be encountered after treating CE patients is the risk of postsurgical relapses or treatment failure, a long-term clinical and serological follow-up is required to evaluate the success and failure of therapy. Materials and Methods We used e...